MAM

Biotechnology Research Teams

Vaccine Technologies Research Team

Vaccine Technologies Research Team conducts studies based on the development of protective and immunotherapeutic treatment methods against biological agents of zoonotic origin or directly affecting human health. Our laboratory has the appropriate infrastructure and expert staff for vaccine development studies in inactive, subunit, recombinant peptide form. In vitro and in vivo studies carried out meticulously in this context include the production of conventional and recombinant toxins, preclinical laboratory-scale vaccine production, examination of vaccine-based humoral and cellular immune responses, modeling of next generation immunotherapeutics, the use of drug and gene-loaded nanocarriers and effective adjuvant systems, development of vaccine-adjuvant formulations and tissue engineering studies. The Vaccine Technologies Research Team contributes to the field of CBRN defense with the development of diagnostic and treatment methods against biological agents and works in line with the needs of public and private sector organizations by providing national/international collaborations.

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Areas of Activity and Work

In vitro Cell Culture

Food toxicology, nanotoxicology, biocompatibility, bioefficacy and biosafety determination, anti-microbial and anti-cancer efficacy.

Tissue Engineering, 3D in vitro Modeling, in vitro Immunotherapeutic Modeling

Cell- and molecular-based immunoregulation in inflammatory disease models

Purification and Chemical Synthesis Studies

Peptide synthesis, purification and characterization.

Vaccine-Adjuvant Studies

Stability, antigen-adjuvant compatibility, immunogenicity & dose, innate and adaptive immune response studies.

In Vivo Animal Modeling and Challenge Studies

Mice, rats, guinea pigs, rabbits and ferrets.

Plant Biotechnology Research Team

The Plant Biotechnology Research Team continues its activities within the scope of producing products and services that can be transformed into added value by using up-to-date biotechnology and genetic engineering methods in line with the country's priorities.

The Plant Biotechnology Research Team collaborates with public research institutions, universities and the private sector at national and international level. In addition, analysis and consultancy services are provided to public and private sector organizations.

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Areas of Activity and Work

Next Generation Genomic Research

Characterization of national plant genetic resources, studies on increasing productivity and quality values in plants with molecular approaches, elucidation of molecular mechanisms involved in biotic / abiotic stress in plants and identification of molecular markers, molecular marker-assisted breeding programs in plants.

Genomic rearrangements in plants

Plant cell suspension and tissue culture applications, production of valuable plant metabolites in plant-based sustainable systems (such as bioreactors) and regulation of their quantities by genetic manipulation.

RNAi

The use of microbial agents in the treatment and prevention of plant diseases.

Biomaterials Research Team

Biomaterials Research Team is a multidisciplinary research team that aims to develop innovative solutions in biomaterials and biotechnology applications. Our team works towards the realization of technologies, products and services with high competitiveness and added value that our country needs in various disciplines such as health, food safety and environmental protection. Our research includes studies on new functional biomaterials for therapeutic and diagnostic purposes, as well as drug delivery systems, development of synthetic drugs and imaging agents, biosensor technologies and diagnostic kits that offer innovative approaches in the early detection and treatment of diseases. We also provide innovative solutions in the field of tissue engineering and regenerative medicine by focusing on the imitation of natural processes through biomimetic and regenerative medicine research, while integrating nanostructures and smart materials in the field of nanotechnology and materials science into healthcare technologies.

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Areas of Activity and Work

For the Treatment of Diseases

Development of synthetic drug molecules, nanocarrier systems, drug formulations and targeted therapeutic agents.

Detection of Diseases and Infections

Biosensor and Diagnostic Kits, Pet imaging agents development studies.

Nanotechnology and Materials Science

Tissue Engineering and biomimetic materials, Antimicrobial Coatings, Nanocomposite Biomaterials, Nanofiber Mask Filters, Antimicrobial Nanofibers, Antimicrobial Hydrogels, 3D Bioprinting Technology, Bioink, Wound dressings, Artificial Skin Graft, Smart Food Packaging and Smart Packaging Labels.

Animal Biotechnology Research Team

The Animal Biotechnology Research Team has achieved important milestones by successfully producing Turkey's first clone calves and first transgenic mice (mice synthesizing human interferon, frost-resistant mice). It conducts genetic and genomic studies in farm animals, domestic and domestic animals, reproductive biology in animals, development of transgenic and experimental animal models, cancer molecular biology, identification of anticancer drug candidates, stem cell therapies, integrated omics analysis for disease diagnosis and treatment, and biodefense product development. It provides services in the field of toxicity and biocompatibility studies in experimental animals by collaborating with public research institutions, universities and private sector at national and international level.

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Areas of Activity and Work

Animal Reproductive Biotechnology Studies and Development of Molecular Breeding Strategies in Livestock and Poultry

Cloning, in vitro fertilization, cryobiology, biobanking, genetic selection, etc.

Animal Genetics Studies and Pedigree Analysis

Identification of genes associated with economically important traits in farm animals, diagnosis of animal diseases such as BLAD, DUMPS where a single mutation is responsible, etc.

Cell Signaling Pathways (Apoptosis, Angiogenesis, Autophagy etc.), Molecular Cancer Biology, Anticancer Drug Development and Embryonic, Mesenchymal and Cancer Stem Cell Studies

Anti-Serum Production and Purification Studies against Pandemic Potential and Disease Causing Agents

Toxicity and Biocompatibility Studies in Experimental Animals, Development of Transgenic and Experimental Animal Models, In Vitro Model Development for Drug Development and Toxicology Studies

Pharmaceutical Technologies Research Team

The Pharmaceutical Technologies Research Team has been competent in antibody engineering, cell line development and characterization, photodynamic therapy, nanoscale drug systems, physico-chemical characterization of proteins and biological activity assays for more than 25 years. In the field of healthcare, the pharmaceutical technologies research team continues to work with different approaches in the discovery of original and biosimilar antibodies, development of new biopharmaceutical drugs, biosimilar mAbs and theranotherapeutic agents, with a priority on cancer and pathogen-induced diseases. The team carries out nationally and internationally supported multidisciplinary projects such as the development of neutralizing antibodies against Sars-CoV-2 virus, the development of the first nationally supported biosimilar antibody for cancer treatment, the production and application of photodynamic therapy agents for cancer diagnosis and treatment, and the development of nanoparticle-based molecules.

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Areas of Activity and Work

Development and production of new drugs and biosimilars based on recombinant antibodies and peptides

Production in mammalian and bacterial cells.

Development of a Biotechnological Drug Producing Cell Line

Research cell bank, master cell bank and working cell bank.

Purification and Physico-Chemical Characterization of Antibodies

HPLC-based analyses (SEC, variant analysis, reverse phase, etc.), FPLC, Capillary Electrophoresis (CE-SDS, CE-IEF), SPR (Surface plasmon resonance) based affinity/kinetic biosimilarity analyses, CD, Zeta Sizer, FTIR, SDS-PAGE, Western, ELISA.

In Vitro Activity Determination of Therapeutics

Inhibition of cell proliferation/IC50/EC50 determination, cell migration/migration and invasion, Apoptosis/ROS, Wound healing assays.

3d Cell Culture, In Vitro Disease Models

Cancer, alzeimer, Type II diabetes, etc.

Directed Immunotherapeutic Applications

Screening Antibody and Peptide Libraries Against Target Antigens with Phage Display Technology

Development of Immunized, Naïve and Synthetic Human or Mouse-Based Antibody Libraries

Human and Humanoid Recombinant Antibody Engineering and Applications in Different Formats (Fab, Scfv, Nanobody, Vh, Vl)

Computer-Based Antibody Humanization

Development of Biologically Activated Materials, Implants, Nanoparticles

Determination of Virus Titer, Detection and Quantification of Neutralizing Antibodies and Antiviral Drug Screening Using the Bsl-3 Substructure

Molecular Oncology Research Team

The Molecular Oncology Research Team offers research on treatment and diagnostic methods especially for cancer, the disease of our age. Recently, it has been working on the development of diagnosis, detection and treatment systems for lung and hematologic cancers. Within the team, a bio-bank has been established from solid and liquid tissue cancer patient samples, and screening, early diagnosis, monitoring and treatment systems have been developed. Using antibodies, immuno-histochemistry kits and immunoassay systems have been developed to identify patients who are candidates for immunotherapy. A therapeutic system was also developed for the treatment of bone defects using mesenchymal stem cells and an osteomimetic hydrogel. One of the main areas of activity of the team is the development of personalized treatment systems. In this context, in addition to developing a CAR-T cell therapy system, we also aim to develop biological drugs, vaccines, small molecules and RNA therapeutics against target molecules and pathways that we have identified in collaboration with relevant expert teams within our unit and outside the institution.

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Areas of Activity and Work

In Vitro Cell Culture and Lentivirus Studies

Production in mammalian and bacterial cells.

Cell Bank

Research cell bank, master cell bank and working cell bank.

Development of Chimeric Antigen Receptor (Car)-T Cell Therapy Systems

Design and expansion of CAR-T GMP-grade T cells using the ClinMACS prodigy cell production system.

Cloning Studies

Fluorescence, near infrared fluorescence and cloning of target molecules into desired cell lines or primary cells for in vitro and in vivo studies

Crispr/Cas9 Studies

TRAC locus targeted system design to prevent GvH reaction, especially in allogeneic CAR-T studies.

Whole Genome, Whole Exome and Total Transcriptome Sequencing, Proteome and Metabolome Studies and Bioinformatics Analysis on Lung Cancer Patient Samples